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insects so dark and dense that even when cleared in
xylol they obstruct the light and are visible only as
silhouettes. Such specimens are often improved by
bleaching. Naturally colorless and very thin-skinned
specimens may become invisible when cleared.
These should be dyed. The stain used to color these
specimens will not “take” unless they also have been
treated briefly with a bleaching solution, so the very
dark and the very light require the same kind of
special treatment before the dehydration begins.
The bleach commonly used for this purpose in
laboratories is a 10% aqueous solution of sodium
hydroxide. Common household “Chlorox,” which is
a 5% aqueous solution of sodium hypochlorite, does
about as well. Put a small quantity of the bleaching
solution in a glass dish, and place the dish on a white
paper so that can see the specimen easily. Transfer
the specimen to this solution from the first alcohol
bath. Watch it closely, through the microscope if
possible. Great care is necessary to avoid over
bleaching. When the appendages become pale and
easily movable, it is time to stop. Further treatment
will dissolve the membranes of the joints, and the
insect will fall apart. Rinse the specimen in clear
water to remove the bleach, then return to dilute
alcohol and continue the preparation as with un-
bleached specimens. Bubbles of gas are sometimes
formed inside of the body during bleaching. These
will probably dissolve before the preparation is com-
peted. If still present when the insect reaches the
final bath, squeeze the abdomen gently to force the
bubbles out through the needle hole.
There are many stains which may be used to tint
colorless specimens. Basic fuchsin is one of the more
commonly used. It is a clear purple-red dye which
you can buy, ready to use, from a scientific supply
house. Apply it to specimens which have been
bleached and run up to 95% alcohol. Put a little of
the dye in a small container and allow the specimen to
soak in it for a minute or two. Rinse in 95% alcohol,
If the insect is not bright pink all over, dip it again.
but do not permit it to become too dark. After dying
and rinsing in alcohol, continue the preparation with
carbol-xylene, xylene, and balsam, as has already
been described.
After seeing what this method of slide preparation
can do, you might like to try more complicated and
professional ones. For expert advice, consult an
article called “Mounting Aphids and Other Small
Insects on Microscope Slides” by E. O. Essig, which
was published in the January, 1948, issue of the
“Pan-Pacific Entomologist. If you do not have access
to a museum or university library which subscribes to
this journal, you can obtain a copy for seventy-five
cents by writing to the California Academy of Sciences,
San Francisco 18. California.
DRY MOUNTING BY CHEMICAL
DEHYDRATION
There are many large soft-bodied arthropods
which cannot readily be inflated. Some of these can
be dry mounted by replacing their natural moisture
with a liquid which so hardens the tissues that they
shrink but little when that liquid is removed. To
minimize distortion, the replacement is accomplished
by degrees, delicate and succulent specimens pro-
gressing more gradually than leathery ones. The
simplest method is to remove the water with alcohol
and the alcohol with xylene, exactly as though the
animal were to be slide-mounted. Xylene evaporates
rapidly, leaving the specimens dry, rigid, and usually
unwrinkled, but somewhat smaller than in life. Al-
though a pattern may persist, color will be almost
wholly lost. Species which are translucent when
living come out perfectly opaque.
The reagents used to dehydrate large arthropods
are those used in slide preparation: 70 and 95% alcohol,
carbol-xylene, and xylol.
Additional equipment includes:
1. Stender dishes or small covered jars, 2 or 3
inches deep and big enough around to accommodate
the outstretched legs of your largest specimen. If you
are preparing one specimen only, one dish will do.
Change the liquids. If you are going to mount a
series, provide one dish for each bath, and transfer
the specimens.
2. Insect or dressmaker's pins, and if possible,
some needle points or “minuten nadeln.”
3. A few pieces of cork or balsa wood about 1/8”
thick and small enough to fit easily into the dishes.
4. Forceps, with which to handle the specimens
and the small pins.
5. Oil colors, for restoring the pigmentation of the
finished product.
Reagents, dishes, and other equipment, if not
obtainable locally, should be ordered from a reliable
scientific supply house.
As an example of the process, let us suppose that
you wish to mount a large spider in a walking position.
First, observe how it stands in life, the height of the
body from the ground and the attitude of the legs.
Then, kill the specimen by dropping it into boiling
water for about half a minute. Spread out the body on
a chip of balsa wood and pose it carefully, bracing and
supporting it with pins as shown in Fig. 12. During
the process of dehydration, the chip floats up-side-
down on the surface of the liquid, a fact which must be
taken into account when placing the pins. When the
specimen is secure against drooping, even in an in-
verted position, fill a dish with 70% alcohol. Float
the chip, face down, upon the surface. Put on the lid,
and leave it over night.
On the following day, remove the float from the