Field notes, v1389
Page 119
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Transcription
P.A.Kelly 1984 27 Journal Procedure for taking tissues: Kill animals in chloroform killing pot. Take measurements - HB, Tail, H.Foot, Ear and WT. Sex & Refro.Cond.. Open Cavity by longitudinal incision from pelvis to sternum. Separate skin from peritoneal muscle. Cut away large oval patch of peritoneal muscle. Remove alimentary tract Paper onto tissues without soiling the coat. Add a drop or two of Heparin to the body cavity to prevent coagulation. Remove one kidney, part of liver, piece of skeletal muscle and heart & freeze in labelled vane tubes. Collect all blood with a heparinized pipette and transfer to & hcp. conical centrifuge tubes and leave on ice until ready to spin down a batch. Clean out the body cavity and pack with paper tissues. label the carcass, place in a plastic bag and freeze. Bushy-Tails killed for all tissues:- 7 PK 109 ♀ (#2128) U.Spring Metofane Overdose, Oct 27, 250g 8 PK 110 ♀ (nullif.) Mile 4.2 333 - 146 - 43 - 32 = 240 g * CUT SHORT 28 PK 111 ♀ (Parson) #2805 L.Sp. 326 - 117 - 43 - 35 = 320g 28 PK 112 ♀ (nullif.) #2169 L.Sp. 315 - 133 - 44 - 32 = 172g Tagged Bushy-Tails at Sagaben Ear Tag # Sex Wt. Location Released Captured Toe Clip. 2207 ♂ 420g U.Sp. April 21,'84 (K 10/28/'84) 2205 ♀ (? ,280g,320g) (U.Sp, R.Sp, L.Sp.) 4/21/'84 9/30/'84, 10/27/'84 94 ♀ U.Sp., Rolide c.6/16/'84 4/20/'84 0002 91 (damiled) (subadult) Rolide c.6/16/'84 0005