Sunday Sept. 16. Staining in Lab. 1. Methylene blue stain (do not know concentration). put Electra in m.b. in oven at temp. 25°C. (at Woods Hole G. had m.b. at 30°C & got good results); leave in over 5 mins (ca 10 mins - here I am trying to recall the time G. put material in oven & then got it out). Heat helps oxidation process wh. is what is involved in m.b. Place on slide, then coverslip, seawater w. pipette if nec. not more than 5-10 mins before animals are dead. G. finds if orient specimen under dissecting scope with bright mirror reflected reaction, the m.b. stain is better. 2. View species from dorsal side this is the only orientation by means of wh. you can see funiculus. We viewed funiculus conc. around brown bodies - degenerating polypide provides nutrients (proteins etc.) wh. are discharged into funicular strands wh. are v. clear near brown bodies. There were cross canals closer to wall betw. funicular strands. G. commented that this was like a primitive circulation system. (contd over)