-3- The muscles of the operculum & diaphragm are on the tentacular sheaths. Stains for whole mounts are haemalén, carmine and haematoxylin. Found a zoecium in Electra (always mounted so you saw the basal side) wh. had no polypide - something had happened so there was no differentiation but there were cells w. nuclei throughout wall. However, there was a pore plate that was differentiating - G.L. indicated that organism will always differentiate around pore plates even if polypide development is suppressed. differentiated envelope. pore plate With methylene blue organism takes up m.b. then dies just as m.b. taken up in nerve. There are 3 processes (sequential) 1) 2) 3) oxidation of m.b. so it shows up as blue stain