BIBBLE, D.Q. 1968 Peromyscus californicus #435 Hastings Reservation, Monterey Co., Calif May 23 Vulva crusty. Vaginal smear: Mostly cornified cells, but poor smear May 24 weight = 39.5 grams. Imperforate - "pin hole" present. Vaginal smear: Only cornified cells seen; slide marginal. May 27 weight = 39.5 grams. Appeared to be perforate. & found in small hollow log placed in cage on 5-26. Given 1/2 apple. Vaginal smear: Primarily Leucocytes present with some (c5%) cornified epithelial cells. Also some nucleated epithelial cells present. May 28 weight = 39.5 grams. Perforate. Both animals found in hollow log. Vaginal smear: ~40% leucocytes, ~40% epithelial cells of a few cornified cells. wt=39.8 grams. Perforate. Both animals in log. Vaginal smear collected: ~50% leucocytes ~50% cornified epithelial cells. Hopefully, She is going through an estrus. Added handful of rolled oats May 30 wt = 39.0 grams. Barely perforate. Vaginal smear collected: poor, but present as ~80% leucocytes and 40% cornified epithelial cells, no nucleated epidermal cells present. May 31 wt = 40.0 grams. Imperforate. Vaginal smear: poor -- mostly cornified cells but a few epithelial cells are present. June 1 wt = 40.0 grams. Imperforate. Vaginal smear: ~95% epithelial cells + 5% leucocytes. Wrapping cotton around bothifice seems to aid in collection of sample, June 2 wt = 39.5 grams. Imperforate. Vaginal smear: ~75% cornified cells + 25% epithelial cells. Added 1/2 apple, some carrot pieces + some spinach greens, June 3 wt = 40.0 grams. Imperforate. Vaginal smear: ~50% cornified + 50% epithelial cells. Not many cells appeared on slide although the swab contained a considerable amount of thick, whitish fluid present in vagina. Cleaned cage & this time added some fresh grass, leaves and chemise. Both animals are definitely spending time in the hollow log nest. June 4 wt = 39.0 grams. Imperforate. Vaginal smear; similar to yesterday's, both in quality smear and quantity of cells present. The female is becoming very difficult to capture and I wonder if the trauma is affecting her ability to reproduce. They are using the log nest, but it does make it more difficult to capture the animals, June 5 wt = 39.0 grams. Imperforate. Vaginal smear: ~95% cornified + 5% epithelial cells. Given 1/4 apple, some green + carrot pieces. June 6 wt = 39.0 grams. Imperforate. Vaginal smear: almost all cornified cells, but again, not many cells total. Vagina still has milky secretion present. June 7 wt = 39.5 grams. Imperforate. Vaginal smear: ~60% leucocytes, 35% cornified cells + 5% epithelial cells. Smear quality very good. NO vaginal secretion evident. June 11 wt = 40.5g. Imperforate. June 15 wt = 43.0 g. Imperforate. June 18 wt = 47.0 g. She either getting very fat or is pregnant! June 21 wt = 47.0 g. "" Given fresh greens, apple, carrot (Going three eats at amazing speed...) June 23 wt = 50.0 g. Cleared cage today. June 25 1630h wt=51g. Pregnant - imperforate. June 26 1230h wt=51g.