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Transcription
killing jar, and clap on the lid. If you are dexterous
with the forceps, you may prefer to use it for handling
dangerous species, as well as those too small or too
inaccessible to be siezed between the finger and
thumb.
Creatures too soft-boiled to dry without distortion
must be collected and preserved in liquid. These
include all spiders, the eggs, larvae and pupae of most
insects, and the adults of some. A few hard-bodied
insects that are black or dark brown in color, such as
dung and water beetles, can stand wetting and may be
collected in liquid and subsequently removed, pinned,
and dried in the usual manner. Hairy, scaley, or
delicate-winged species are collected in liquid only
when wanted for dissection, as their appearance is
irreparably ruined by such treatment. All species,
hard or soft, which are so small that they must be
mounted on microscope slides are properly collected
wet. Caterpillars which are to be inflated can be col-
lected in liquid, but the color will be better if they are
brought into the laboratory alive and dropped into
boiling water, or gasses in the dry killing jar, like
a hard-bodied insect, and blown immediately there-
after.
A very large number of preserving fluids have been
concocted by learned collectors, each claiming some
particular advantage. Their formulae are available in
technical publications. This museum, however, uses
nothing but 95% pure ethyl alcohol, somewhat diluted
with water for most purposes. Unfortunately for the
amateur entomologist, pure ethyl alcohol, 190 proof
grain neutral spirits, is subject to a federal liquor tax
which makes its price prohibitively high. Its sale is
further restricted by State regulations. In New York
State it cannot be purchased without a Doctor's
perscription or a permit from the State Liquor Au-
thority. Many scientific institutions, such as university
laboratories, hospitals, and museums, have permits,
and are also tax exempt, but they may be called to
account for every pint they use. If you can arrange to
obtain your alcohol from such a source, you are in
luck. If you cannot, go to the nearest drug store and
find a rubbing-alcohol compound containing 70% of
ethyl alcohol. This will be a satisfactory killing fluid
for all but the most delicate species. As a preservative,
it is just barely strong enough. When a small bottle-
full is diluted with the body fluids of several large
specimens, it ceases to be effective, and must be re-
placed. Change the alcohol on even a few small
specimens at least once, that on a large lot two or
three times, within a few days of capture. Thereafter,
r renew the liquid whenever it becomes discolored.
For very tiny thin-skinned species, such as aphids
and trips, use a killing fluid containing, by volume,
one part of water to three of 70% alcohol, replacing it
with straight 70% alcohol the following day. This
"stepping up" minimizes the shrinkage caused by
sudden removal of water from the tissues.
"Completely denatured alcohol" contains 95% of
ethyl alcohol, rendered im potable by the addition of
a little wood alcohol and gasoline. You can buy it
at many large drug stores, or order it from a chemical
supply house, and dilute it as you would pure alcohol to
serve your several purposes. To make a killing fluid
for delicate specimens, add 6 parts of water to 7 of
alcohol. For general collecting, and to preserve very
small species, use 21/2 parts water to 7 of alcohol; and
for preserving those of large or moderate size, 2 to 11.
Always replace the killing fluid by the more concen-
trated preserving fluid within a day or two. The
stronger the alcohol, the less frequently must it be
changed because of dilution by the natural moisture of
the specimens, but too strong a mixture will make the
creatures brittle. Laboratory procedures for dealing
with soft-bodied arthropods are discussed in Leaflet
Number 6.
To look well, a collection of alcoholic specimens
should be stored in bottles of uniform design and
a limited number of sizes. Those used as standard
in this Museum are lipped vials with corks, 2 dram size
for small and medium sized species, 4 dram for large
ones. Such vials are obtainable at drug stores and
scientific supply houses. When purchased in quantity,
the price is surprisingly low. Doubling as collecting
bottles, a pocketfull of these little storage vials have
several advantages over a single large collecting jar.
For one thing, you cannot accidentally spill your whole
day's supply of alcohol at one time. For another, each
species, colony, or associated group of creatures can
have a bottle and label to itself. Do not forget the
label, date, locality and observations, written in soft
pencil on a small slip of paper and placed inside the
bottle with the specimens. Color notes will be
particularly valuable, as vivid or delicate hues are
bleached by alcohol, while creatures which are pale or
colorless gradually turn dark brown.
Both the discoloration and the distortion of liquid
preservation will be less if you bring back your
specimens alive, and kill them by stewing in water
heated to just below the boiling point. Let them
simmer for five to fifteen minutes, according to size.
Then, if they are large enough to handle, puncture
each with a needle, to relieve internal pressure, before
transferring them to alcohol. Stewing is highly
recommended by specialists in the study of larvae,
particularly for the preparation of grubs and other
wax-white animals. When the beauty of the specimens
is more important than economy of time, it certainly
repays the extra effort.
If a tight-corked bottle is more than three quarters
full of liquid, the air pressure caused by the insertion
of the stopper may be great enough to force it out
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