Field notes, v1524
Page 401
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Transcription
AKP 1973 had just sighted them and was depart after them with a gun. OPart I had breakfast and then started picking the legs of the T Phyllotis for EM comparisons with those from near Jaena. We also had 2 Jaena Phyllotis with us which we fixed at the same time. Not too happy with the fixation method - but we shall see. We have been - etherizing the animal, exposing the trachea and introducing some fixative there. When he has stopped breathing, we open the chest cut either the aorta or (hopefully through st. ventricle) the vena cava, perfuse first a little Ringer's (reptilia (?) and then fixative until the heart stops beating. The perfusion apparatus I had rigged up has been not very satisfactory etc etc etc. Then I have excised 2 pieces of lung tissues (for the same sites fixation: overnight in all animals) and put it in fresh fixative overnight. up in an oil Really, have needed a better apparatus or a board for holding the for 2 hrs. - animal. 2Os - 0.1M CaCO3. 3 hrs in UAc, dehydrate. Dave shot a tinamou for us, which we roasted on a spit and ate at tea time. The weather has been closing in on us here as at Chalapalca, but somehow never seems so windy or cold. Monday March 26 Yante caƱ This morning started embueding the lung tissues. OPart put another "integrator" into a leg just as we spent the day down at the spring watching the operated lizard and catching some others to take back to Benest.